Leveraging the CRSPR/Cas9 technology, EdiGene has developed and validated over 2,000 ready-made Knockout聽 Cell Lines聽(Edi-G KO Cell Lines)聽and our list is continuously expanding. 聽For each knockout cell line, we also have developed its derivative products such as Knockout聽Cell Lysates(Edi-G KO Cell Lysates)and knockout total RNAs. The knockout cell derivatives provide further options and flexibility for the genome editing workflow. In addition to being very powerful tools for identifying, validating and confirming gene functions in聽cancer biology聽and signaling pathways, Edi-G KO Cell Lines and Lysates聽are fast becoming industry leading standards for antibody-validation聽and quality control.


Edi-G KO Advantages:

  • Unlike many other ready-made knockout cells, Edi-G knockouts are created in the most commonly used cell lines such as HeLa,聽HEK293T,聽HCT116, HepG2, A549 and Jurket聽cells etc.
  • Currently, we have over 2,000 validated, ready-made Edi-G KO Cell Lines with complete loss of gene function available for your selection.
  • Sequencing validation: 聽The target region of each Edi-G KO Cell Line is sequenced twice to ensure each allele of the target gene has a insertion/deletion mutation but not in-frame mutation.
  • Each validated sgRNA is available for purchase separately so that you may perform your own gene knockout in the cells of your choice.
  • To further simplify and save you time, we provide Edi-G KO Cell Derivative Products including cell lysates, cell pellets, and total RNAs for immediate use.

Edi-G KO Applications:

Edi-G KO Cell Lines and Cell Derivative Products are ideally suited for:

聽 聽 路 聽Gene function and biological pathway analysis
聽 聽 路 聽Biomarker discovery and validation
聽 聽 路 聽Drug target identification and validation
聽 聽 路 聽Cancer development and progression studies
聽 聽 路 聽Polyclonal and monoclonal antibody validation

You may also browse聽our KO cell lines and their derivative聽products聽by your research interests in particular signaling pathways and cancer related genes. We have generated knockout cell lines for hot genes such as BRAF, EGFR, KRAS, NRAS, NOTCH1-3, PD-1, PD-L1 PIK3, TP53, etc., popular pathways includingsHippo Signaling Pathway,聽P53 Signaling Pathway,聽Autophagy,聽Apoptosis,聽JAK/STAT Signaling Pathway,聽MAPK Signaling Pathway,聽PI3K-AKT Signaling Pathway, and cancer related genes including聽Acute Myeloid Leukemia,聽Breast Cancer,聽Chronic Myeloid Leukemia,聽Colorectal Cancer,聽Melanoma,聽Non-Small Cell Lung Cancer,聽Pancreatic Cancer,聽Small Cell Lung Cancer, etc.

If you cannot find the KO line in our database, please contact us for custom cell engineering services.